RESEARCH ON INDIGENOUS MICROBE MOLECULE ECOLOGY OF DAN-12 BLOCK IN SHENGLI OILFIELD

In order to recognize the exchanges of indigenous microbe ecology in reservoir,established DGGE and T-RFLP analysis method based on increase of PCR.Extracted all DNA of microbe of injected and produced well of Dan12 block in shengli oilfield,and extend 16S rDNA based on it,and do DGGE and T-RFLP analysis.The DGGE analysis results show that inject water and Dan 12-4 well are all have 10 stripes,Dan 12-5 have 7 stripes.But the superiority strips of Dan 12-4 and 12-5 are all 6 stripes,great than 4 stripes of inject water.The T-RFLP analysis shows that there is desulphovibrio,pseudomonad,immobilize-bacillus and clostridium,they are all useful for oil production.Researched the change discipline of reservoir microbe in view of molecule ecology,it is important to optimize microbe oil displacement scheme and enhance displacement efficiency.