Abstract: To accurately understand the physiological biochemical characteristics of production fungus can provide theoretical basis for the paulopost fungus preservation, the cultivation and selection of fungi more suitable for microbiological production. In this paper, the morphologic observation of colonies and thalli of 74 fungi preserved in the lab is conducted. The gram-chromatic classification is made. Analysis and measurement is conducted on the physiological biochemical characteristics of bacterial strains including the carbohydrate fermentation experiment, the anaerobic nitrate gas producing experiment, the hydrogen sulphide producing experiment, and the kinematic performance. Results show that most of the 74 fungi belong to gram-positive fungi, while some of them are anaerobic ones and feature in acid production, no hydrogen sulphide production, good kinematic performance, anaerobic nitrate gas production. All the features are favorable for microbiological production.