Abstract: A suit of heat and pressure preservation sampling system was designed in this paper, which is suitable for high sulfur gas wells in 6500m depth. It is made up of downhole sampling device (DSD) and ground transfer device (GTD), and can collect natural gas in high sulfur natural gas wells and long-time heat and pressure maintaining storage and transportation. So the using of this system can preserve real physicochemical properties of samples and enhance the effectiveness of PVT experiments. A clock controller was adopted in DSD to control automatic collection and record the state parameters in whole course and the vacuum heat preservation interlayer structure and heat insulation coating technique were used to ensure heat and pressure preservation function. Meanwhile, for convenient transferring, the structure of DSD is divided into two demountable parts: electric control chamber and gas sampling chamber. GTD is in a seal cylinder structure equipped with electric control system which possesses gas collection chambers maintaining heat and pressure storage and transportation for long time and H2S leaking emergency treatment abilities. In conclusion, the equipment is featured by compact structure and complete function. It can provide a suit of safe and reliable equipment for sampling in deep and high sulfur gas wells.